1 tube            

10 tubes

20 tubes

10*RT buffer

2

20

40

dNTP (4 uM)

3

30

60

primer

1

10

20

DMSO

3 

30

60

H₂O

4.5

45

90

RNAse             

0.5 

5

10

RT (10 units)

1

10

20

RNA

5

Total

20 ul

200 ul

400 ul