Agarose Gel Electrophoresis

A 2% agarose gel with ethidium bromide is used:

1.    Weigh 6g of agarose and pour into 300 ml of 1*TBE

2.    Mix and microwave for 5 minutes using a high setting

3.    Allow to cool to about 60^oC, then add 15 ml of 10ug/ml ethidium bromide

4.    Mix thoroughly and pour gel into gel holder

5.    After the gel has set, dry load the samples onto the gel

6.    Place holder into the tank, make sure the buffer is just above the bottom of the gel.

7.    Electrophorese at 150V for 2 minutes to run the samples into the gel

8.    Add more buffer until the whole gel is covered.

9.    Electrophorese at 150V for 10 minutes